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Photomed Laser Surg. 2011 Dec;29(12):827-33. doi: 10.1089/pho.2011.3022. Epub 2011 Sep 14.

Effectiveness of photodynamic therapy for the inactivation of Candida spp. on dentures: in vitro study.

Author information

1
Dentistry School, São Francisco University, Bragança Paulista, SP, Brazil.

Abstract

OBJECTIVE:

This in vitro study evaluated the effectiveness of photodynamic therapy (PDT) for the inactivation of different species of Candida on maxillary complete dentures.

BACKGROUND DATA:

The treatment of denture stomatitis requires the inactivation of Candida spp. on dentures. PDT has been reported as an effective method for Candida inactivation.

METHODS:

Reference strains of C. albicans, C. glabrata, C. tropicalis, C. dubliniensis and C. krusei were tested. Thirty-four dentures were fabricated in a standardized procedure and subjected to ethylene oxide sterilization. The dentures were individually inoculated with one of the strains and incubated at 37°C for 24 h. Dentures submitted to PDT (P+L+) were individually sprayed with 50 mg/L of Photogem(®) (PS) and, after 30 min, illuminated by LED light for 26 min (37.5 J/cm(2)). Additional dentures were treated only with PS (P+L-) or light (P-L+) or neither (P-L-). Samples of serial dilutions were spread on Sabouraud dextrose agar and incubated at 37°C for 48 h. The colonies were counted and the values of log (cfu/mL) were analyzed by Kruskall-Wallis and Dunn tests (p<0.05).

RESULTS:

For all species of Candida, PDT resulted in significant reduction (p<0.05) of cfu/mL values from dentures when compared with P-L- (reductions from 1.73 to 3.99 log(10)). Significant differences (p<0.05), but lower reductions, were also observed for P+L- and P-L+when compared with P-L- for some species of Candida.

CONCLUSIONS:

PDT was an effective method for reducing Candida spp. on dentures.

PMID:
21916614
PMCID:
PMC3231796
DOI:
10.1089/pho.2011.3022
[Indexed for MEDLINE]
Free PMC Article

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