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J Ethnopharmacol. 2011 Oct 31;138(1):85-91. doi: 10.1016/j.jep.2011.08.048. Epub 2011 Sep 1.

Neuroprotective effect of Alpinia galanga (L.) fractions on Aβ(25-35) induced amnesia in mice.

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1
Department of Pharmacology, Lalitha College of Pharmacy, Ghatkesar, Gr. Hyderabad, Andhra Pradesh 501301, India.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The rhizomes of Alpinia galanga (L.) Willd (Zingiberaceae), a ginger substitute for flavouring food was traditionally used as nervine tonic and stimulant.

AIM OF THE STUDY:

This investigation is designed to screen cognitive improvement of Alpinia galanga (AG) fractions in Alzheimer's type of amnesia in mice induced by Aβ((25-35)).

MATERIALS AND METHODS:

Alzheimer's disease induced mice treated with fractions (n-hexane, chloroform and ethyl acetate) of AG in 200 and 400mg/kg. Neurotoxicity was induced by intracerebroventricular injection of Aβ((25-35)) on the 14th day of 21 days drug treatment. Open field and water maze were carried to determine habituation memory and hippocampal memory. Na(+)/K(+)-ATPase, acetylcholinesterase (AChE) and antioxidant enzymes (SOD, GPx, catalase and vitamin C) were determined in brain tissue homogenate to estimate the brain biochemical changes and its anti-amnesic potential with intensity of oxidative stress signaling. Further bioactive (chloroform) fraction was eluted through column chromatography to identify the lead molecules.

RESULTS:

Increased habituation memory and decreased escape latency in behavioral parameter are the indicative of the cognitive enhancement after treatment with Alpinia galanga fractions. Increment in Na(+)/K(+)-ATPase and antioxidant activity depicts brain membrane integrity improvement and free radical scavenging property. AChE level was decreased to improve the cognition by enhancing cholinergic transmission.

CONCLUSION:

Anti-amnesic effect was exerted by various fractions of Alpinia galanga. Among all fractions, preeminent neuroprotection was exerted by chloroform fraction, which has compound, 1'δ-1'-acetoxyeugenol acetate and it may be a potential therapeutic agent for Alzheimer's type of amnesia. These results further motivate us to explore the activity of lead compound's anti-amnesic effect on transgenic mice model of AD.

PMID:
21911048
DOI:
10.1016/j.jep.2011.08.048
[Indexed for MEDLINE]

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