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Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.

Light-based feedback for controlling intracellular signaling dynamics.

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1
Cardiovascular Research Institute and Department of Biochemistry, University of California San Francisco, San Francisco, California, USA.

Abstract

The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.

PMID:
21909100
PMCID:
PMC3184382
DOI:
10.1038/nmeth.1700
[Indexed for MEDLINE]
Free PMC Article
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