Send to

Choose Destination
Metab Eng. 2011 Nov;13(6):656-65. doi: 10.1016/j.ymben.2011.08.002. Epub 2011 Sep 1.

Mapping photoautotrophic metabolism with isotopically nonstationary (13)C flux analysis.

Author information

Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235, USA.

Erratum in

  • Metab Eng. 2012 Mar;14(2):185.


Understanding in vivo regulation of photoautotrophic metabolism is important for identifying strategies to improve photosynthetic efficiency or re-route carbon fluxes to desirable end products. We have developed an approach to reconstruct comprehensive flux maps of photoautotrophic metabolism by computational analysis of dynamic isotope labeling measurements and have applied it to determine metabolic pathway fluxes in the cyanobacterium Synechocystis sp. PCC6803. Comparison to a theoretically predicted flux map revealed inefficiencies in photosynthesis due to oxidative pentose phosphate pathway and malic enzyme activity, despite negligible photorespiration. This approach has potential to fill important gaps in our understanding of how carbon and energy flows are systemically regulated in cyanobacteria, plants, and algae.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center