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Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592. Epub 2011 Sep 8.

An expanded palette of genetically encoded Ca²⁺ indicators.

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1
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.

Abstract

Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca(2+)) concentration are powerful tools for visualizing intracellular signaling activity. However, despite a decade of availability, the palette of single FP-based Ca(2+) indicators has remained limited to a single green hue. We have expanded this palette by developing blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change. This series enables improved single-color Ca(2+) imaging in neurons and transgenic Caenorhabditis elegans. In HeLa cells, Ca(2+) was imaged in three subcellular compartments, and, in conjunction with a cyan FP-yellow FP-based indicator, Ca(2+) and adenosine 5'-triphosphate were simultaneously imaged. This palette of indicators paints the way to a colorful new era of Ca(2+) imaging.

PMID:
21903779
PMCID:
PMC3560286
DOI:
10.1126/science.1208592
[Indexed for MEDLINE]
Free PMC Article
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