Format

Send to

Choose Destination
Tissue Eng Part C Methods. 2011 Dec;17(12):1161-70. doi: 10.1089/ten.TEC.2011.0201. Epub 2011 Sep 14.

Evaluation of a thin and mechanically stable collagen cell carrier.

Author information

1
Institute of Anatomy, Center for Regenerative Biology and Medicine, Eberhard Karls University, Tuebingen, Germany.

Abstract

The biological function of adherent cell populations strongly depends on the physical and biochemical properties of extracellular matrix molecules. Therefore, numerous biocompatible cell carriers have been developed to specifically influence cell attachment, proliferation, cellular differentiation, and tissue formation for diverse cell culture applications and cell-based therapies. In the present study, we evaluated the mechanical and the cell biological properties of a novel, thin, and planar collagen scaffold. The cell carrier is based on fibrillar bovine collagen type I and exhibits a low material thickness coupled with a high mechanical stability as measured by tensile tests. The influence of this new biomaterial on cell viability, proliferation, and cell differentiation was analyzed using 5-bromo-2-deoxyuridine (BrdU) proliferation assay, immunocytochemistry, water-soluble tetrazolium salt-1 assay (WST-1), live cell imaging, and electron microscopy. Cell culture experiments with the human osteosarcoma cell line Saos-2, human mesenchymal stem cells, and rodent cardiomyocytes demonstrated the in vitro biocompatibility of this chemically noncrosslinked scaffold. Both the mechanical characteristics and the in vitro biocompatibility of this collagen type I carrier facilitate the engineering of thin transferable tissue constructs and offer new possibilities in the fields of cell culture techniques, tissue engineering, and regenerative medicine.

PMID:
21902619
PMCID:
PMC3226420
DOI:
10.1089/ten.TEC.2011.0201
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center