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Int J Oncol. 2012 Feb;40(2):402-8. doi: 10.3892/ijo.2011.1191. Epub 2011 Sep 7.

Fibulin-3 promoter methylation alters the invasive behavior of non-small cell lung cancer cell lines via MMP-7 and MMP-2 regulation.

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Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, 1045 Daedeok-Daero, Yusong-gu, Daejeon 305-353, Republic of Korea.


Fibulin-3, an extracellular glycoprotein, has been suggested as having functions in tissue regeneration and organogenesis. However, its role in cancer remains unclear. We show here that fibulin-3 was silenced by hypermethylation of the promoter region in the relatively invasive A549 non-small cell lung cancer (NSCLC) cells compared with less invasive H460 NSCLC cells. Enforced expression of fibulin-3 in A549 cells down-regulated cellular MMP-7 and MMP-2, which was followed by inhibition of cell invasiveness. Conversely, suppression of fibulin-3 expression with siRNA in H460 cells showed the opposite effect. These results indicate that fibulin-3 is a negative regulator of invasiveness in NSCLC and further studies are needed for its therapeutic applications in treatment of NSCLC.

[Indexed for MEDLINE]

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