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J Sep Sci. 2011 Oct;34(19):2739-44. doi: 10.1002/jssc.201100452. Epub 2011 Aug 24.

Preparation and evaluation of molecularly imprinted microspheres for solid-phase extraction of 1,4-hydroxybenzoic acid esters in soy.

Author information

1
School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, P. R. China. juanhe@haut.edu.cn

Abstract

Molecularly imprinted microspheres (MIMs) were prepared by precipitation polymerization for the binding and recognition of 1,4-hydroxybenzoic acid esters. Ethyl p-hydroxybenzoate (EtPHB) was used as the template molecule, methacrylic acid as the functional monomer, ethylene dimethacrylate as the linking agent. It was evaluated by solid-phase extraction column packed with MIMs combined with liquid chromatography to determine trace preservatives including benzoic acid, methyl p-hydroxybenzoate, EtPHB, propyl p-hydroxybenzoate in food products. A solid-phase extraction based on MIM procedure was used to isolate four additives from the food matrix before quantitative analysis. The Scatchard plot suggested that the template-polymer system had two-site binding behavior with the dissociation constants of 0.3577 and 3.952 mg/g, respectively. The rebinding test, based on the molecularly imprinted solid-phase extraction column technique, showed the recoveries of soy samples spiked with four additives within 88.4-110.6%, with the relative standard deviations of 1.97-3.82%. Finally, the method was successfully applied for the analysis of parabens in foodstuff without traditional pretreatment.

PMID:
21898813
DOI:
10.1002/jssc.201100452
[Indexed for MEDLINE]

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