Abstract
This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amifostine / pharmacology
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Animals
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Antioxidants / pharmacology
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Caspase 3 / metabolism
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Caspase 3 / radiation effects
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Catechin / analogs & derivatives
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Catechin / pharmacology
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Cell Survival / radiation effects
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Cells, Cultured / cytology
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Cells, Cultured / enzymology
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Cells, Cultured / radiation effects*
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Dose-Response Relationship, Radiation
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Female
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Gamma Rays*
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Hippocampus / cytology
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Hippocampus / enzymology
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Hippocampus / radiation effects*
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L-Lactate Dehydrogenase / radiation effects
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Neurons / cytology
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Neurons / enzymology
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Neurons / radiation effects*
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Poly(ADP-ribose) Polymerases / drug effects
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Pregnancy
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Rats
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Rats, Sprague-Dawley
Substances
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Antioxidants
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Catechin
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epigallocatechin gallate
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L-Lactate Dehydrogenase
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Poly(ADP-ribose) Polymerases
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Caspase 3
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Amifostine