Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):16038-43. doi: 10.1073/pnas.1111650108. Epub 2011 Sep 6.

Arbovirus high fidelity variant loses fitness in mosquitoes and mice.

Author information

1
Institut Pasteur, Viral Populations and Pathogenesis, Centre National de la Recherche Scientifique, Unité de Recherche Associée 3015, 75724 Paris Cedex 15, France.

Abstract

The error rate of RNA-dependent RNA polymerases (RdRp) affects the mutation frequency in a population of viral RNAs. Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity and generates populations with reduced genetic diversity. In mosquitoes, high fidelity CHIKV presents lower infection and dissemination titers than wild type. In newborn mice, high fidelity CHIKV produces truncated viremias and lower organ titers. These results indicate that increased replication fidelity and reduced genetic diversity negatively impact arbovirus fitness in invertebrate and vertebrate hosts.

PMID:
21896755
PMCID:
PMC3179076
DOI:
10.1073/pnas.1111650108
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center