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J Eukaryot Microbiol. 2011 Sep-Oct;58(5):463-8. doi: 10.1111/j.1550-7408.2011.00574.x. Epub 2011 Sep 2.

Naegleria fowleri: enolase is expressed during cyst differentiation.

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1
Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Avenue IPN 2508, Zacatenco, 07360 Mexico City, Mexico. bchavez@cinvestav.mx

Abstract

Cysts of Naegleria fowleri present an external single-layered cyst wall. To date, little information exists on the biochemical components of this cyst wall. Knowledge of the cyst wall composition is important to understand its resistance capacity under adverse environmental conditions. We have used of a monoclonal antibody (B4F2 mAb) that specifically recognizes enolase in the cyst wall of Entamoeba invadens. By Western blot assays this antibody recognized in soluble extracts of N. fowleri cysts a 48-kDa protein with similar molecular weight to the enolase reported in E. invadens cysts. Immunofluorescence with the B4F2 mAb revealed positive cytoplasmic vesicles in encysting amebas, as well as a positive reaction at the cell wall of mature cysts. Immunoelectron microscopy using the same monoclonal antibody confirmed the presence of enolase in the cell wall of N. fowleri cysts and in cytoplasmic vesicular structures. In addition, the B4F2 mAb had a clear inhibitory effect on encystation of N. fowleri.

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