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J Biomed Opt. 2011 Aug;16(8):086006. doi: 10.1117/1.3606574.

Transverse chemical interface detection with coherent anti-Stokes Raman scattering microscopy.

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  • 1Aix-Marseille Université, Domaine Universitaire Saint-Jéro^me, Institut Fresnel, Mosaic group, CNRS UMR 6133, École Centrale de Marseille, 13397 Marseille cedex 20, France.


Transverse "chemical" interfaces are revealed with a conventional two beam narrowband coherent anti-Stokes Raman scattering microscopy setup in a collinear configuration. The exciting "pump" and "Stokes" beams are focused on the sample in two opposite directions. The subtraction of the two generated anti-Stokes signals gives rise to a signal that is directly proportional to the pure Raman spectrum of the resonant medium. This property is used to highlight an interface between glass and N,N-dimethylformamide (DMF) and recover the pure Raman spectrum of DMF around its 1408 cm(-1) vibrational band.

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