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J Bacteriol. 1990 Jun;172(6):3500-2.

Rule governing the division pattern in Escherichia coli minB and wild-type filaments.

Author information

1
Institut Jacques Monod, Université Paris 7, France.

Abstract

Escherichia coli minB mutants form anucleate minicells and multinucleate filaments. We show here that the overwhelming majority of nucleate cells contain 2n (n = 0, 1, 2, ...) nucleoids, as determined by 4',6-diamidino-2-phenylindole staining, and 2n (n = 1, 2, 3, ...) copies of the replication origin, as determined by flow cytometry. This shows that division sites are not chosen randomly among the available sites in minB filaments. Similarly, wild-type cells contain 2n nucleoids, both during cell division inhibition and when furazlocillin-induced filaments are allowed to divide. We conclude that the min+ function is only to prevent septation only at polar sites; the placement of internal cell division sites must obey strict rules, which are the same in minB and wild-type cells.

PMID:
2188963
PMCID:
PMC209166
[Indexed for MEDLINE]
Free PMC Article

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