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FEBS Lett. 2011 Oct 3;585(19):3026-32. doi: 10.1016/j.febslet.2011.08.023. Epub 2011 Aug 27.

Role of Sch9 in regulating Ras-cAMP signal pathway in Saccharomyces cerevisiae.

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  • 1Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.


In Saccharomyces cerevisiae PKA plays a major role in regulating cell growth, metabolism, and stress resistance. We report that Sch9 regulates PKA directly and SCH9 deletion enhances PKA activity by showing that: (1) Bcy1 predominately localized in the nucleus in glycerol-grown sch9Δ cells; (2) large part of the catalytic subunits of PKA transferred from the nucleus to the cytoplasm in sch9Δ cells; (3) higher protein stability of Tpk2 resulted in higher protein level of Tpk2 in sch9Δ than in wild type cells. Our investigations suggest that Sch9 regulates phosphorylation of Bcy1. We also observed hyper-phosphorylation of Cdc25 in sch9Δ, in contrast to the tpk2Δ and tpk2Δsch9Δ mutants, suggesting that feedback inhibition of PKA on Cdc25 is through Tpk2.

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