Format

Send to

Choose Destination
Clin Lab. 2011;57(7-8):489-95.

Inactivation of herpes simplex virus type 1 & adenovirus type 5 by direct electric current at a biocompatible level in vitro.

Author information

1
Department of Biophysics, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran. Roohandeh@yahoo.com

Abstract

BACKGROUND:

In vitro studies were conducted to quantify the effectiveness of low-level direct electric current (DC) on infectivity of Herpes Simplex Virus type 1 (HSV-1), Adenovirus type 5 (AdV-5), and on viability of Vero cells.

METHODS:

Both viruses and Vero cells were exposed to DC by using platinum electrodes connected to a DC source, then the viral infectivity and cell viability were assessed by plaque and MTT assay, respectively.

RESULTS:

The results showed that both viruses were inactivated completely by 200 microA DC in 10 minutes (current density = 20 microA/mm2) while this amount of DC had no significant changes on the viability of Vero cells (viability > 90 %). Inactivation degree of HSV-1 and AdV-5 was 5 and 4 log per mL, respectively. Further study is required to investigate the mechanism of inactivation by this method.

CONCLUSIONS:

DC at a biocompatible level showed the competency to inactivate the viruses in the solution completely. So it is a useful tool for designing a noninvasive method for decontamination of biological or synthetic fluids. This method or derivation can be considered as an easy, fast, and economical method for fluid decontamination.

PMID:
21888012
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center