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Sleep. 2011 Sep 1;34(9):1261-9. doi: 10.5665/SLEEP.1252.

Sleep disturbance impairs stroke recovery in the rat.

Author information

1
Neurocenter of Southern Switzerland, Lugano, Switzerland.

Abstract

STUDY OBJECTIVES:

There is a lack of experimental evidence to support the hypothesis that sleep may modulate stroke outcome as suggested by clinical observations. We have previously shown that sleep disturbance (SDis) over 3 days aggravates brain damage in a rat model of focal cerebral ischemia. The aim of this study is to further investigate effects of SDis on long-term stroke recovery and neuroplasticity as assessed by axonal sprouting, neurogenesis, and angiogenesis.

DESIGN:

Focal cerebral ischemia was induced by permanent occlusion of the distal branches of middle cerebral artery. Twelve hours after initiation of ischemia, SDis was performed over 3 consecutive days (deprivation of 80% sleep during the 12-h light phase). Weekly assessments on sensorimotor function by the single pellet reaching test (SPR) were performed for 5 weeks after surgery. Axonal sprouting was evaluated by anterograde tracing with biotinylated dextran amine (BDA) and neurogenesis/angiogenesis by bromodeoxyuridine (BrdU) labelling along with cell-type markers. Control groups included ischemia without SDis, sham with SDis, and sham without SDis.

SETTING:

Basic sleep research laboratory.

MEASUREMENTS AND RESULTS:

Rats subjected to SDis after ischemia showed significantly less recovery of forearm motor skills during the post-stroke period of 5 weeks. This effect was accompanied by a substantial reduction in axonal sprouting, expression of synaptophysin, and the ischemia-stimulated neural and vascular cell proliferation.

CONCLUSION:

SDis has detrimental effects on functional and morphological/structural outcomes after stroke, suggesting a role of sleep in the modulation of recovery processes and neuroplasticity.

KEYWORDS:

Stroke; axonal sprouting; brain repair; neurogenesis; neuroplasticity; sleep; sleep deprivation

PMID:
21886364
PMCID:
PMC3157668
DOI:
10.5665/SLEEP.1252
[Indexed for MEDLINE]
Free PMC Article
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