Xpert MTB/RIF for rapid diagnosis of tuberculous lymphadenitis from fine-needle-aspiration biopsy specimens

Louis J Ligthelm; Mark P Nicol; Kim G P Hoek; Rachael Jacobson; Paul D van Helden; Ben J Marais; Robin M Warren; Colleen A Wright

doi:10.1128/JCM.01310-11

Xpert MTB/RIF for rapid diagnosis of tuberculous lymphadenitis from fine-needle-aspiration biopsy specimens

J Clin Microbiol. 2011 Nov;49(11):3967-70. doi: 10.1128/JCM.01310-11. Epub 2011 Aug 31.

Authors

Louis J Ligthelm¹, Mark P Nicol, Kim G P Hoek, Rachael Jacobson, Paul D van Helden, Ben J Marais, Robin M Warren, Colleen A Wright

Affiliation

¹ Division of Anatomical Pathology, National Health Laboratory Service, Tygerberg Hospital, Tygerberg, Cape Town, South Africa. louisligthelm@sun.ac.za

Abstract

This study demonstrates the excellent diagnostic accuracy of the Xpert MTB/RIF test in patients with tuberculous lymphadenitis. The test sensitivity and specificity were 96.7% (95% confidence interval [CI], 86.6 to 100%) and 88.9% (95% CI, 69.6 to 100%), respectively, and it correctly identified 6/6 (100%) of the cytology smear-negative/culture-positive cases and 1 of 2 (50%) rifampin-resistant cases.

Publication types

Evaluation Study

MeSH terms

Adolescent
Adult
Antitubercular Agents / pharmacology
Bacteriological Techniques / methods*
Biopsy, Fine-Needle*
Child
Child, Preschool
Drug Resistance, Bacterial*
Female
Humans
Male
Molecular Diagnostic Techniques / methods*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Reagent Kits, Diagnostic
Rifampin / pharmacology
Sensitivity and Specificity
Tuberculosis, Lymph Node / diagnosis*
Young Adult

Substances

Antitubercular Agents
Reagent Kits, Diagnostic
Rifampin