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AIDS Res Hum Retroviruses. 1990 Apr;6(4):465-79.

Escherichia coli expression, purification, and biological activity of a truncated soluble CD4.

Author information

1
Bioprocess Research & Development, Upjohn Company, Kalamazoo, MI 49001.

Abstract

A truncated molecule containing the N-terminal 183 amino acid residues of CD4 (sCD4-183) has been produced in Escherichia coli at high levels, using the trp promoter and an AT-rich ribosome binding site to direct expression in a pBR322-derived vector. A culture has been selected which allows large-scale fermentation and production of this material as an insoluble inclusion body protein. Procedures which solubilize, refold, and purify sCD4-183 have been developed. The purified sCD4-183 binds gp120 in solution and blocks human immunodeficiency virus (HIV) infection of human peripheral blood lymphocytes in vitro.

PMID:
2187501
DOI:
10.1089/aid.1990.6.465
[Indexed for MEDLINE]

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