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Methods Mol Biol. 2011;763:73-81. doi: 10.1007/978-1-61779-191-8_4.

Epidermal permeability barrier measurement in mammalian skin.

Author information

1
Department of Pharmaceutical Sciences, College of Pharmacy, and Environmental Health Sciences Center, Oregon State University, Corvallis, OR, USA. Arup.Indra@oregonstate.edu

Abstract

A defective skin epidermal permeability barrier (EPB) is responsible for a high mortality rate in premature infants and is an important risk factor in inflammatory skin diseases such as eczema. We report here fast and accurate methods for measurement of EPB in animal models or in human patients using simple techniques that monitor diffusion of dyes (X-Gal or Lucifer Yellow) through the upper epidermis and measure transepidermal water loss (TEWL) resulting from a defective skin barrier. Accurate diagnosis and early detection of EPB defects in human patients are critical for effective treatment of certain classes of inflammatory skin diseases.

PMID:
21874444
PMCID:
PMC3306247
DOI:
10.1007/978-1-61779-191-8_4
[Indexed for MEDLINE]
Free PMC Article

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