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Nat Methods. 2011 Aug 28;8(10):849-51. doi: 10.1038/nmeth.1679.

Stable-isotope labeling with amino acids in nematodes.

Author information

1
Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK.

Abstract

We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

PMID:
21874007
PMCID:
PMC3184259
DOI:
10.1038/nmeth.1679
[Indexed for MEDLINE]
Free PMC Article

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