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Nat Methods. 2011 Aug 28;8(10):845-7. doi: 10.1038/nmeth.1675.

Quantitative proteomics by amino acid labeling in C. elegans.

Author information

1
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

Abstract

We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.

PMID:
21874006
DOI:
10.1038/nmeth.1675
[Indexed for MEDLINE]

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