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Methods Mol Biol. 2011;756:285-94. doi: 10.1007/978-1-61779-160-4_16.

Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activity.

Author information

1
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Abstract

The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.

PMID:
21870233
PMCID:
PMC4386889
DOI:
10.1007/978-1-61779-160-4_16
[Indexed for MEDLINE]
Free PMC Article

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