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Transgenic Res. 2012 Jun;21(3):665-9. doi: 10.1007/s11248-011-9545-3. Epub 2011 Aug 26.

Conditional Allele Mouse Planner (CAMP): software to facilitate the planning and design of breeding strategies involving mice with conditional alleles.

Author information

1
Epithelial Systems Biology Group, Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, 6N312, Bethesda, MD 20892, USA.

Abstract

Transgenic and conditional knockout mouse models play an important role in biomedical research and their use has grown exponentially in the last 5-10 years. Generating conditional knockouts often requires breeding multiple alleles onto the background of a single mouse or group of mice. Breeding these mice depends on parental genotype, litter size, transmission frequency, and the number of breeding rounds. Therefore, a well planned breeding strategy is critical for keeping costs to a minimum. However, designing a viable breeding strategy can be challenging. With so many different variables this would be an ideal task for a computer program. To facilitate this process, we created a Java-based program called Conditional Allele Mouse Planner (CAMP). CAMP is designed to provide an estimate of the number of breeders, amount of time, and costs associated with generating mice of a particular genotype. We provide a description of CAMP, how to use it, and offer it freely as an application.

PMID:
21870117
DOI:
10.1007/s11248-011-9545-3
[Indexed for MEDLINE]

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