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Biochem Biophys Res Commun. 2011 Sep 9;412(4):723-7. doi: 10.1016/j.bbrc.2011.08.040. Epub 2011 Aug 17.

Extremely low polymerizability of a highly-divergent Chlamydomonas actin (NAP).

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Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Tokyo, Japan.


Novel actin-like protein (NAP) is a highly divergent actin expressed in Chlamydomonas. With its low sequence similarity, it is uncertain whether NAP can polymerize into filaments. Here I assessed it by ectopically expressing enhanced green fluorescent protein-tagged NAP (EGFP-NAP) in cultured cells. EGFP-NAP was excluded from stress fibres but partially co-localized with endogenous actin in the cell periphery. In fluorescence recovery after photobleaching experiment, turnover rate of EGFP-NAP was similar to the estimated diffusion rate of monomeric actin. Therefore, EGFP-NAP likely accumulates by diffusion. These findings suggest that NAP has extremely poor ability to polymerize.

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