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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Aug;19(4):999-1004.

[Evaluation of methods for detection of NPM1 gene mutations in acute myeloid leukemia].

[Article in Chinese]

Author information

1
School of Life Science, Xiamen University, Xiamen 361005, Fujian Province, China.

Abstract

The purpose of this study was to establish real-time based methods for detection of NPM1 gene mutation in acute myeloid leukemia (AML). Primers/probes were designed according to the clustered region of NPM1 mutations on exon 12. Two real-time PCR assays, including high resolution melting curve (HRM) and allele-specific PCR (AS-PCR), were developed and clinically evaluated with 89 AML samples, which were parallelly detected by capillary electrophoresis (CE) and sequencing. The results showed that a total of 17 mutation-positive samples were detected, including type A (15 cases), type B (1 case) and type Nm (1 case). HRM assay could detect all mutant types, and the analytical sensitivity was around 5%. In contrast, AS-PCR assay detected only 95% mutant types, but its sensitivity was as high as 0.01%. It is concluded that considering the characteristics of each method as well as the clinical evaluation results, HRM may be used for screening of NPM1 mutations at diagnosis, while the AS-PCR can be used for the MRD quantification during follow-up.

PMID:
21867632
[Indexed for MEDLINE]

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