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Eur J Clin Microbiol Infect Dis. 2012 May;31(5):835-9. doi: 10.1007/s10096-011-1382-z. Epub 2011 Aug 25.

A comparison of the Sensititre® MYCOTB panel and the agar proportion method for the susceptibility testing of Mycobacterium tuberculosis.

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1
Department of Pediatric Infectious Diseases, Mount Sinai School of Medicine, Mount Sinai Medical Center, 1 Gustave L. Levy Place, Box 1657, New York, NY 10029, USA. mayssa.abuali@gmail.com

Abstract

The agar proportion method (APM) for determining Mycobacterium tuberculosis susceptibilities is a qualitative method that requires 21 days in order to produce the results. The Sensititre method allows for a quantitative assessment. Our objective was to compare the accuracy, time to results, and ease of use of the Sensititre method to the APM. 7H10 plates in the APM and 96-well microtiter dry MYCOTB panels containing 12 antibiotics at full dilution ranges in the Sensititre method were inoculated with M. tuberculosis and read for colony growth. Thirty-seven clinical isolates were tested using both methods and 26 challenge strains of blinded susceptibilities were tested using the Sensititre method only. The Sensititre method displayed 99.3% concordance with the APM. The APM provided reliable results on day 21, whereas the Sensititre method displayed consistent results by day 10. The Sensititre method provides a more rapid, quantitative, and efficient method of testing both first- and second-line drugs when compared to the gold standard. It will give clinicians a sense of the degree of susceptibility, thus, guiding the therapeutic decision-making process. Furthermore, the microwell plate format without the need for instrumentation will allow its use in resource-poor settings.

PMID:
21866324
DOI:
10.1007/s10096-011-1382-z
[Indexed for MEDLINE]

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