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Nucleic Acids Res. 1990 Apr 11;18(7):1687-91.

DNA recombination during PCR.

Author information

1
Laboratoire de Biologie et Immunologie Mol├ęculaires des R├ętrovirus, Institut Pasteur, Paris, France.

Abstract

PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.

PMID:
2186361
PMCID:
PMC330584
DOI:
10.1093/nar/18.7.1687
[Indexed for MEDLINE]
Free PMC Article

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