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Eur J Nutr. 2012 Aug;51(5):563-72. doi: 10.1007/s00394-011-0240-1. Epub 2011 Aug 24.

Aronia melanocarpa fruit extract exhibits anti-inflammatory activity in human aortic endothelial cells.

Author information

1
Department of Biochemistry and Clinical Chemistry, The Warsaw Medical University, Banacha 1 Street, 02-097, Warsaw, Poland. zapolska@vp.pl

Abstract

BACKGROUND:

Altered expression of cell adhesion molecules (CAMs) has been implicated in a variety of chronic inflammatory conditions, including atherosclerosis. Regulation of adhesion molecule expression by specific redox-sensitive mechanisms has been reported. Additionally, it has been observed that the extract of Aronia melanocarpa (A. Melanocarpa) fruits, rich in polyphenols, exhibits potent anti-oxidant properties and displays cardioprotective activity.

METHODS AND RESULTS:

Human aortic endothelial cells (HAECs) were pretreated with various concentrations (primarily 50 μg/mL) of Aronia Melanocarpa fruit extract prior to treatment with TNFα (10 ng/mL) for various periods of time. The surface protein and mRNA expression of ICAM-1 and VCAM-1 were determined using flow cytometry and real-time RT-PCR, respectively. Adhesion of peripheral blood mononuclear leucocytes (PBMLs) to TNFα-treated HAECs was evaluated by an adhesion assay. Activation of NF-κB was evaluated by measuring NF-κB p65 phosphorylation using flow cytometry. ROS production was determined by reduction in fluorescent 2',7'-dichlorofluorescein diacetate (DCFH-DA). Tested A. Melanocarpa extract significantly inhibited the expression of ICAM-1 and VCAM-1, attenuated the phosphorylation of NF-κB p65 and decreased intracellular ROS production in TNFα-treated HAECs.

CONCLUSION:

We conclude that A. Melanocarpa fruit extract exhibits anti-inflammatory effects in HAECs by inhibiting the expression of endothelial CAMs, activation of NF-κB and production of ROS.

PMID:
21863241
PMCID:
PMC3397226
DOI:
10.1007/s00394-011-0240-1
[Indexed for MEDLINE]
Free PMC Article

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