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Curr Opin Genet Dev. 2011 Oct;21(5):558-65. doi: 10.1016/j.gde.2011.07.003. Epub 2011 Aug 19.

Shedding light on the system: studying embryonic development with light sheet microscopy.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA. tomerr@janelia.hhmi.org

Abstract

Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications. There is an outstanding potential in applying this technology to the quantitative study of embryonic development. Here, we provide an overview of the different basic implementations of LSFM, review recent technical advances in the field and highlight applications in the context of embryonic development. We conclude with a discussion of promising future directions.

PMID:
21862314
DOI:
10.1016/j.gde.2011.07.003
[Indexed for MEDLINE]

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