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Indian J Med Microbiol. 2011 Jul-Sep;29(3):254-7. doi: 10.4103/0255-0857.83908.

Molecular characterization of CTX-Mβ-lactamases among Klebsiella pneumoniae isolated from patients at Tehran hospitals.

Author information

1
Department of Microbiology, Pasteur Institute, Tehran, Iran.

Erratum in

  • Indian J Med Microbiol. 2011 Oct-Dec;29(4):449. Shoeib, N [corrected to Nematzadeh, Shoeib]; Fereshteh, S [corrected to Shahcheraghi, Fereshteh]; Mehdi, F M [corrected to Feizabadi, Mohammad Mehdi]; Sadat, N V [corrected to Nikbin, Vajiheh Sadat ]; Leila, N [corrected to Nasehi, Leila].

Abstract

PURPOSE:

Plasmid-encoded CTX-M-group of extended-spectrum β-lactamases (ESBLs) represent a significant and rapidly emerging problem in most part of the world. The aim of the present study was to describe the prevalence of CTX-M producing Klebsiella pneumoniae at Tehran hospitals.

MATERIALS AND METHODS:

Clinical isolates of K. pneumoniae (n=250) were collected from 10 hospitals of Tehran. Susceptibility to antimicrobial agents, MIC of cefotaxime and ESBLs production of collected isolates were detected. All ESBL-producing isolates were screened for bla CTX-M genes using PCR and DNA sequencing. Molecular typing of bla(CTX-M) harboring isolates was performed by Pulsed-field gel electrophoresis assay.

RESULTS:

Of 250 K. pneumoniae clinical isolates, 102 isolates revealed ESBLs - phenotype. PCR assay and sequencing detected bla(CTX-M) genes in 71.5% (n= 73) of ESBL-producing isolates. The prevalence of CTX-M -I and CTX-M-III clusters among these isolates was 35.61% (n=26) and 21.9 % (n=16) respectively. Coexistence of CTX-M -I and CTX-M-III clusters was found among 42.5% (n= 31) of isolates. Of 102 isolates that were positive in the phenotypic confirmatory test (PCT), 29 isolates (28.4%) did not produce any amplicons in PCR for bla(CTX-M) gene. The results of PCR for CTX-M -II and CTX-M-IV clusters were also negative. Analysis of the 31 CTX-M producing K. pneumoniae isolates by PFGE typing showed 26 distinct patterns.

CONCLUSIONS:

The bla CTX-M genes are widespread among Iranian isolates of K. pneumoniae. PFGE demonstrated the high diversity of K. pneumoniae harboring bla(CTX-M) in our study.

PMID:
21860105
DOI:
10.4103/0255-0857.83908
[Indexed for MEDLINE]
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