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PLoS One. 2011;6(8):e22956. doi: 10.1371/journal.pone.0022956. Epub 2011 Aug 8.

Drosophila Ten-m and filamin affect motor neuron growth cone guidance.

Author information

1
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America.

Abstract

The Drosophila Ten-m (also called Tenascin-major, or odd Oz (odz)) gene has been associated with a pair-rule phenotype. We identified and characterized new alleles of Drosophila Ten-m to establish that this gene is not responsible for segmentation defects but rather causes defects in motor neuron axon routing. In Ten-m mutants the inter-segmental nerve (ISN) often crosses segment boundaries and fasciculates with the ISN in the adjacent segment. Ten-m is expressed in the central nervous system and epidermal stripes during the stages when the growth cones of the neurons that form the ISN navigate to their targets. Over-expression of Ten-m in epidermal cells also leads to ISN misrouting. We also found that Filamin, an actin binding protein, physically interacts with the Ten-m protein. Mutations in cheerio, which encodes Filamin, cause defects in motor neuron axon routing like those of Ten-m. During embryonic development, the expression of Filamin and Ten-m partially overlap in ectodermal cells. These results suggest that Ten-m and Filamin in epidermal cells might together influence growth cone progression.

PMID:
21857973
PMCID:
PMC3152545
DOI:
10.1371/journal.pone.0022956
[Indexed for MEDLINE]
Free PMC Article

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