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Biomed Microdevices. 2012 Feb;14(1):7-16. doi: 10.1007/s10544-011-9580-0.

Dual microfluidic perifusion networks for concurrent islet perifusion and optical imaging.

Author information

1
Department of Transplant/Surgery, University of Illinois at Chicago, 840 South Wood Street, Clinical Sciences Building, Suite 402, Chicago, IL 60612, USA.

Abstract

This study explores a new class of duplex microfluidic device which utilizes a dual perifusion network to simultaneously perform live-cell optical imaging of physiological activities and study insulin release kinetics on two islet populations. This device also incorporates on-chip staggered herringbone mixers (SHMs) to increase mixing efficiency and facilitate the generation of user-defined chemical gradients. Mouse islets are used to simultaneously measure dynamic insulin release, changes in mitochondrial potentials, and calcium influx in response to insulin secretagogues (glucose and tolbutamide), and show a high signal-to-noise ratio and spatiotemporal resolution of all measured parameters for both perifusion chambers. This system has many potential applications for studying β-cell physiology and pathophysiology, as well as for therapeutic drug screening. This dual perifusion device is not limited to islet studies and could easily be applied to other tissues and cells without major modifications.

PMID:
21850483
PMCID:
PMC3696955
DOI:
10.1007/s10544-011-9580-0
[Indexed for MEDLINE]
Free PMC Article

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