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Pak J Biol Sci. 2010 May 15;13(10):504-8.

Improved production of L-lysine by over-expression of Meso-diaminopimelate decarboxylase enzyme of Corynebacterium glutamicum in Escherichia coli.

Author information

1
Department of Biochemistry, Payam-E-Noor University, Tehran, Iran.

Abstract

The aim of this study is over-expression of Meso-diaminopimelate decarboxylase enzyme (EC 4.1.1.20) and enhancement of L-lysine production rate. The C. glutamicum LysA gene which encodes a Meso-diaminopimelate decarboxylase was cloned in E. coli. The cloned gene was sequenced; it encodes a 445 amino acids protein with molecular weight of 47 kD. Expression of the LysA gene in E. coli resulted in an increase in Meso-diaminopimelate decarboxylase activity, correlated with the presence in sodium dodecyl sulfate-polyacrylamid gels of a clear protein band that corresponds to this enzyme. The induction of cloned gene by IPTG has been shown to have an inhibitory effect on cell growth due to over-expression of the cloned gene. A two fold increase in lysine production rate was observed after introduction of the cloned gene into E. coli.

PMID:
21848075
[Indexed for MEDLINE]

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