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Biophys J. 2011 Aug 17;101(4):970-5. doi: 10.1016/j.bpj.2011.06.060.

Stoichiometry of reconstituted high-density lipoproteins in the hydrated state determined by photon antibunching.

Author information

1
Physical and Life Science Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA.

Abstract

Apolipoprotein A-I plays a central role in the solution structure of high-density lipoproteins. Determining the stoichiometry of lipid-bound apo A-I in the hydrated state is therefore fundamental to understanding how high-density lipoproteins form and function. Here, we use the quantum optical phenomenon of photon antibunching to determine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obtained by photon-counting histogram analysis. Both the photon antibunching and photon-counting analyses show that reconstituted high-density lipoprotein particles contain two apo A-I molecules, which is in agreement with the commonly accepted double-belt model.

PMID:
21843489
PMCID:
PMC3175089
DOI:
10.1016/j.bpj.2011.06.060
[Indexed for MEDLINE]
Free PMC Article

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