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Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14198-203. doi: 10.1073/pnas.1111704108. Epub 2011 Aug 9.

Multiple new site-specific recombinases for use in manipulating animal genomes.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147-2408, USA.

Abstract

Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools. However, the small number of recombinases demonstrated to have distinct specificities, low toxicity, and sufficient activity to drive reactions to completion in animals has been a limitation. In this report we show that four recombinases derived from yeast--KD, B2, B3, and R--are highly active and nontoxic in Drosophila and that KD, B2, B3, and the widely used FLP recombinase have distinct target specificities. We also show that the KD and B3 recombinases are active in mice.

PMID:
21831835
PMCID:
PMC3161616
DOI:
10.1073/pnas.1111704108
[Indexed for MEDLINE]
Free PMC Article

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