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Inflamm Bowel Dis. 2011 Sep;17(9):1943-54. doi: 10.1002/ibd.21572. Epub 2011 Jan 6.

Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in Crohn's disease.

Author information

1
Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. benjamin.funke@med.uni-heidelberg.de

Abstract

BACKGROUND:

A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD).

METHODS:

The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25).

RESULTS:

Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) within the epithelial layer of the ileum in patients with CD but not with UC. The downregulation of RIG-I was confirmed by quantitative RT-PCR, immunoblot analysis, and immunohistochemistry.

CONCLUSIONS:

Epithelial downregulation of RIG-I, a known pattern recognition receptor for viral components, might contribute to alterations of the innate mucosal immune response, particularly in CD.

PMID:
21830273
DOI:
10.1002/ibd.21572
[Indexed for MEDLINE]
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