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Bioanalysis. 2011 Aug;3(15):1741-51. doi: 10.4155/bio.11.156.

A practical approach to reduce interference due to in-source collision-induced dissociation of acylglucuronides in LC-MS/MS.

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1
Algorithme Pharma Inc, 575 Armand-Frappier, Laval, Québec, Canada, H7V 4B3.

Abstract

BACKGROUND:

In LC-MS/MS, glucuronide conjugated metabolites may convert back to the parent drug due to in-source collision-induced dissociation (CID).

RESULTS:

During the bioanalysis of naproxen, it was noticed that naproxen acylglucuronide exhibited intense in-source CID to the naproxen [M+H](+) ion under positive ESI. However, no in-source CID of the acylglucuronide to the naproxen [M+NH(4)](+) adduct was observed. Furthermore, absolutely no in-source CID was detected under negative ESI. This phenomenon was not only observed for naproxen acylglucuronide but for eight other acylglucuronides compounds.

CONCLUSION:

We have shown that monitoring the parent drug [M-H](-) or [M+NH(4)](+) whenever possible could be an easy approach used by bioanalytical scientists to minimize the impact of in-source CID of acylglucuronides to the parent drug.

PMID:
21827272
DOI:
10.4155/bio.11.156
[Indexed for MEDLINE]
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