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PLoS One. 2011;6(8):e23160. doi: 10.1371/journal.pone.0023160. Epub 2011 Aug 3.

Checkpoints in adenoviral production: cross-contamination and E1A.

Author information

1
Vascular Biology Section, Boston University Medical Center, Boston, Massachusetts, United States of America.

Abstract

Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.

PMID:
21826235
PMCID:
PMC3149638
DOI:
10.1371/journal.pone.0023160
[Indexed for MEDLINE]
Free PMC Article

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