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Cell Res. 2012 Jan;22(1):237-47. doi: 10.1038/cr.2011.127. Epub 2011 Aug 9.

Small molecule-based disruption of the Axin/β-catenin protein complex regulates mesenchymal stem cell differentiation.

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1
Department of Advanced Fermentation Fusion Science & Technology, Kookmin University, Seoul, Korea.

Abstract

The Wnt/β-catenin pathway plays important roles in the differentiation of multiple cell types, including mesenchymal stem cells. Using a cell-based chemical screening assay with a synthetic chemical library of 270 000 compounds, we identified the compound SKL2001 as a novel agonist of the Wnt/β-catenin pathway and uncovered its molecular mechanism of action. SKL2001 upregulated β-catenin responsive transcription by increasing the intracellular β-catenin protein level and inhibited the phosphorylation of β-catenin at residues Ser33/37/Thr41 and Ser45, which would mark it for proteasomal degradation, without affecting CK1 and GSK-3β enzyme activities. Biochemical analysis revealed that SKL2001 disrupted the Axin/β-catenin interaction, which is a critical step for CK1- and GSK-3β-mediated phosphorylation of β-catenin at Ser33/37/Thr41 and Ser45. The treatment of mesenchymal stem cells with SKL2001 promoted osteoblastogenesis and suppressed adipocyte differentiation, both of which were accompanied by the activation of Wnt/β-catenin pathway. Our findings provide a new strategy to regulate mesenchymal stem cell differentiation by modulation of the Wnt/β-catenin pathway.

PMID:
21826110
PMCID:
PMC3351914
DOI:
10.1038/cr.2011.127
[Indexed for MEDLINE]
Free PMC Article
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