Format

Send to

Choose Destination
See comment in PubMed Commons below
Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. doi: 10.1073/pnas.1110854108. Epub 2011 Aug 8.

Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors.

Author information

1
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.

Erratum in

  • Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9219.

Abstract

To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other viruses. The G protein from lymphocytic choriomeningitis virus endowed VSV with the ability to spread transsynaptically, specifically in an anterograde direction, whereas the rabies virus glycoprotein gave a specifically retrograde transsynaptic pattern. The use of an avian G protein fusion allowed specific targeting of cells expressing an avian receptor, which allowed a demonstration of monosynaptic anterograde tracing from defined cells. Synaptic connectivity of pairs of virally labeled cells was demonstrated by using slice cultures and electrophysiology. In vivo infections of several areas in the mouse brain led to the predicted patterns of spread for anterograde or retrograde tracers.

Comment in

PMID:
21825165
PMCID:
PMC3174680
DOI:
10.1073/pnas.1110854108
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center