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Methods Mol Biol. 2011;767:201-20. doi: 10.1007/978-1-61779-201-4_15.

Immunocytochemical analysis of human pluripotent stem cells.

Author information

1
Neuroscience Laboratories, Centers for Neuroscience and Translational Research, CHOC Research Institute, Children's Hospital of Orange County Research Institute, Orange, CA, USA.

Abstract

This chapter will describe the most common immunocytochemical method utilized in the stem cell field - using fluorescently tagged secondary antibodies to detect a primary antibody that is bound to an epitope on a molecule of interest. Secondary antibodies recognize the heavy chain of the primary antibody's isotype. Generally, these methods employ an incubation period of the sample with the primary antibody, a series of washes to remove unbound primary antibody, a secondary incubation period of the sample with the fluorescently conjugated secondary antibody, followed by washes and preparation for microscopy.

PMID:
21822877
PMCID:
PMC3695829
DOI:
10.1007/978-1-61779-201-4_15
[Indexed for MEDLINE]
Free PMC Article

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