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J Ethnopharmacol. 2011 Oct 11;137(3):1197-206. doi: 10.1016/j.jep.2011.07.048. Epub 2011 Aug 2.

Anti-inflammatory activity of ethanol extract derived from Phaseolus angularis beans.

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1
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.

Abstract

ETHNOPHARMACOLOGICAL SIGNIFICANCE:

Phaseolus angularis Wight (adzuki bean) is an ethnopharmacologically well-known folk medicine that is prescribed for infection, edema, and inflammation of the joints, appendix, kidney and bladder in Korea, China and Japan.

AIM OF STUDY:

The anti-inflammatory effect of this plant and its associated molecular mechanisms will be investigated.

MATERIALS AND METHODS:

The immunomodulatory activity of Phaseolus angularis ethanol extract (Pa-EE) in toll like receptor (TLR)-activated macrophages induced by ligands such as lipopolysaccharide (LPS), Poly (I:C), and pam3CSK was investigated by assessing nitric oxide (NO) and prostaglandin (PG)E(2) levels. To identify which transcription factors such as nuclear factor (NF)-κB and their signaling enzymes can be targeted to Pa-EE, biochemical approaches including reporter gene assays, immunoprecipitation, kinase assays, and immunoblot analyses were also employed. Finally, whether Pa-EE was orally available, ethanol (EtOH)/hydrochloric acid (HCl)-induced gastritis model in mice was used.

RESULTS:

Pa-EE dose-dependently suppressed the release of PGE(2) and NO in LPS-, Poly(I:C)-, and pam3CSK-activated macrophages. Pa-EE strongly down-regulated LPS-induced mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Interestingly, Pa-EE markedly inhibited NF-κB, activator protein (AP)-1, and cAMP response element binding protein (CREB) activation; further, according to direct kinase assays and immunoblot analyses, Pa-EE blocked the activation of the upstream signaling molecules spleen tyrosine kinase (Syk), p38, and transforming growth factor β-activated kinase 1 (TAK1). Finally, orally administered Pa-EE clearly ameliorated EtOH/HCl-induced gastritis in mice.

CONCLUSION:

Our results suggest that Pa-EE can be further developed as a promising anti-inflammatory remedy because it targets multiple inflammatory signaling enzymes and transcription factors.

PMID:
21821108
DOI:
10.1016/j.jep.2011.07.048
[Indexed for MEDLINE]

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