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J Ethnopharmacol. 2011 Oct 11;137(3):1077-82. doi: 10.1016/j.jep.2011.07.024. Epub 2011 Jul 28.

Inonotus obliquus extracts suppress antigen-specific IgE production through the modulation of Th1/Th2 cytokines in ovalbumin-sensitized mice.

Author information

1
Lab of Hygienic Pharmacy, College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Youngsan-ku, Seoul 140-742, Republic of Korea.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Chaga mushroom (Inonotus obliquus, IO) has been used as a folk remedy for cancer, digestive system diseases, and other illnesses in Russia and Eastern Europe.

AIM OF THE STUDY:

In the present study, we investigated the immunomodulating effects of IO through in vivo and ex vivo studies.

MATERIALS AND METHODS:

Serum immunoglobulins (IgE, IgG(1), and IgG(2a)) and cytokines (interleukin (IL)-4, interferon (IFN)-γ, and IL-2) were measured in concanavalin A (ConA)-stimulated splenocytes and CD4(+) T cells. The nitric oxide (NO) secretion of lipopolysaccharide (LPS)-stimulated peritoneal macrophages was also measured after oral administration of 50, 100, or 200 mg kg(-1) d(-1) IO hot water extract (IOE) to ovalbumin (OVA)-sensitized BALB/c mice.

RESULTS:

We found that the OVA-induced increase in serum IgE and IgG(2a) was significantly suppressed when IOE was orally administered after the second immunization with OVA. ConA stimulation in spleen cells isolated from OVA-sensitized mice treated with 100 mg kg(-1) IOE resulted in a 25.2% decrease in IL-4 production and a 102.4% increase in IFN-γ, compared to the controls. Moreover, IL-4, IFN-γ, and IL-2 were significantly reduced after ConA stimulation in isolated CD4(+)T cells. We also determined that IOE inhibits the secretion of NO from LPS-stimulated peritoneal macrophages ex vivo.

CONCLUSIONS:

We suggest that IO modulates immune responses through secretion of Th1/Th2 cytokines in immune cells and regulates antigen-specific antibody production.

PMID:
21820502
DOI:
10.1016/j.jep.2011.07.024
[Indexed for MEDLINE]

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