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Dev Cell. 2011 Aug 16;21(2):328-42. doi: 10.1016/j.devcel.2011.06.020. Epub 2011 Aug 4.

Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit.

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1
Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK. p.vagnarelli@ed.ac.uk

Abstract

Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain localizes Repo-Man to bulk chromatin in early anaphase. There, it targets PP1 for the dephosphorylation of histone H3 and possibly other chromosomal substrates. An N-terminal domain localizes Repo-Man to the chromosome periphery later in anaphase. There, it is responsible for the recruitment of nuclear components such as Importin β and Nup153 in a PP1-independent manner. These observations identify Repo-Man as a key factor that coordinates chromatin remodeling and early events of nuclear envelope reformation during mitotic exit.

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PMID:
21820363
PMCID:
PMC3480639
DOI:
10.1016/j.devcel.2011.06.020
[Indexed for MEDLINE]
Free PMC Article
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