Hydroxylation modification and free radical scavenging activity of puerarin-7-O-fructoside

Folia Microbiol (Praha). 2011 Jul;56(4):305-11. doi: 10.1007/s12223-011-0052-y. Epub 2011 Aug 5.

Abstract

Puerarin-7-O-fructoside was transformed by Trichoderma harzianum CGMCC 1523 into 3'-hydroxypuerarin-7-O-fructoside; this was identified by MS and NMR. However, puerarin-7-O-glucoside was not directly hydroxylated but hydrolyzed back into puerarin, which was transformed into 3'-hydroxypuerarin by the same fungi. Comparative analysis of free radical scavenging activity of DPPH showed that the free radical scavenging activity of puerarin-7-O-glucoside was reduced to approximately 1/2 of that of puerarin, while the free radical scavenging activity of puerarin-7-O-fructoside was increased to approximately 1.5 times of that of puerarin. The free radical scavenging activity of 3'-hydroxypuerarin-7-O-fructoside was further increased by 2.2 times of that of puerarin-7-O-fructoside, which was close to that of 3'-hydroxypuerarin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotransformation
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / metabolism*
  • Fructose / analogs & derivatives*
  • Fructose / chemistry
  • Fructose / metabolism
  • Glycosylation
  • Hydroxylation
  • Isoflavones / chemistry
  • Isoflavones / metabolism*
  • Trichoderma / metabolism

Substances

  • Free Radical Scavengers
  • Isoflavones
  • puerarin-7-O-fructoside
  • Fructose