Abstract
A water soluble vitamin B(12)-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.
MeSH terms
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Biological Transport
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Cell Line, Tumor
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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Gene Expression Regulation, Neoplastic
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / pathology*
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Receptors, Cell Surface / genetics*
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Rhenium / chemistry*
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Solubility
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Vitamin B 12 / chemistry*
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Vitamin B 12 / metabolism*
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Water / chemistry*
Substances
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Fluorescent Dyes
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Receptors, Cell Surface
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intrinsic factor-cobalamin receptor
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Water
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Rhenium
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Vitamin B 12