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Methods Mol Biol. 2011;758:49-66. doi: 10.1007/978-1-61779-170-3_4.

Microglia cell culture: a primer for the novice.

Author information

1
Department of Neurology, University of Ulm, Ulm, Germany.

Abstract

Microglial cells are the resident immune cells of the central nervous system. Progress in the recent decade has clearly established that microglial cells participate or even actively drive neurological disease. Much of our current knowledge has been generated by investigating microglial cells in cell culture. The aim of this chapter is to give the uninitiated a basic and adaptable protocol for the culturing of microglial cells. We discuss the challenges of microglial cell culture and provide a collection of tips which reflect our 25+ years of collective experience.

PMID:
21815058
DOI:
10.1007/978-1-61779-170-3_4
[Indexed for MEDLINE]

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