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Urology. 2011 Oct;78(4):792-6. doi: 10.1016/j.urology.2011.05.049. Epub 2011 Aug 2.

Semen parameters and sperm DNA fragmentation as causes of recurrent pregnancy loss.

Author information

1
Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, Sousse, Tunisia. brahemsonia@yahoo.fr

Abstract

OBJECTIVES:

To evaluate and compare standard sperm parameters, and sperm DNA fragmentation in seminal ejaculates from men whose partners had a history of recurrent pregnancy loss (RPL) and in a control group of men who had recently established their fertility.

METHODS:

Semen samples from 31 patients with a history of recurrent pregnancy loss and 20 men with proven fertility were analyzed according to World Health Organization guidelines. Sperm DNA fragmentation was detected by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay.

RESULTS:

When sperm quality of the control group was compared with that of the RPL group, a significant difference was observed in sperm motility, but not in other parameters. The mean number of sperm cells with fragmented DNA was significantly increased in the RPL group (32.22 ± 6.14%) compared with control donors (10.20 ± 2.1%).

CONCLUSIONS:

Our data indicate that sperm from men with a history of RPL have a higher incidence of DNA damage and poor motility than sperm from a control group, and this can explain in part the pregnancy loss in these patients.

PMID:
21813165
DOI:
10.1016/j.urology.2011.05.049
[Indexed for MEDLINE]

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