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Age Ageing. 2011 Sep;40(5):568-75. doi: 10.1093/ageing/afr084. Epub 2011 Aug 1.

Human serum N-glycan profiles are age and sex dependent.

Author information

1
Department of Life Science and Engineering, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, Heilongjiang, China.

Abstract

BACKGROUND:

protein glycosylation varies with the physiological and pathological status of the cell. Consequently, analysis of protein-linked glycans has growing importance both in basic glycobiological research and as a potential tool for monitoring the physiological state in humans.

DESIGN, SETTING AND PARTICIPANTS:

a total of 265 healthy northern Chinese men and women were grouped by age and gender. The mean age in males and females was similar.

OBJECTIVE:

the study is aimed to evaluate the effects of the age and gender on the human serum N-glycans profiles in the clinical diagnose of ageing and disease.

METHODS:

the 265 human serum N-glycan profiles were obtained by DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. Comparison of N-glycan profiles was carried out among the different genders and age groups and the data were analysed with the GeneMapper software.

RESULTS:

age-related changes in the three N-glycan structures (NGA2F, NGA2FB and NA2F) were observed. Interestingly, fucosylation of N-glycans was significantly different (P < 0.0001) between men and women: more core-α-1,6-fucosylated glycans were detected in women, whereas more branching-α-1,3-fucosylated N-glycans were seen in men.

CONCLUSIONS:

the N-glycome profile in serum is gender and age dependent. This should be taken into consideration in the development of serum glycome markers.

PMID:
21807702
DOI:
10.1093/ageing/afr084
[Indexed for MEDLINE]

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