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Cell. 1990 Apr 6;61(1):135-45.

Tubulovesicular processes emerge from trans-Golgi cisternae, extend along microtubules, and interlink adjacent trans-golgi elements into a reticulum.

Author information

1
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510.

Abstract

Morphological dynamics and membrane transport within the living Golgi apparatus of astrocytes labeled with NBD-ceramide were imaged using both electronically enhanced fluorescence video and laser confocal microscopy. In time-lapse recordings, continuous tubulovesicular processes are observed to emerge from trans-Golgi elements and extend along microtubules at average rates of 0.4 microns/s. In addition, discrete fluorescent particles are observed to emerge from the trans-Golgi and subsequently migrate along microtubules at comparable velocities. Frequently, tubulovesicular processes form stable connections that interlink adjacent trans-Golgi elements into an extensive reticulum. Laser photobleaching-recovery experiments reveal that tubulovesicular processes can provide direct pathways for the diffusion of membrane lipids between joined trans-Golgi elements. These results suggest that microtubule-based transport and membrane fusion can operate to interconnect certain cisternal membranes of adjacent Golgi elements within the cell.

PMID:
2180583
DOI:
10.1016/0092-8674(90)90221-y
[Indexed for MEDLINE]

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